Journal of Molecular and Cellular Cardiology
Volume 48, Issue 6 , Pages 1316-1323 , June 2010

PDGF-BB protects cardiomyocytes from apoptosis and improves contractile function of engineered heart tissue

  • Marius Vantler

      Affiliations

    • Klinik III für Innere Medizin, Universität zu Köln, Germany
    • Center for Molecular Medicine (CMMC), Universität zu Köln, Germany
    • These authors contributed equally.
  • ,
  • Bijoy Chandapillai Karikkineth

      Affiliations

    • Institute for Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • These authors contributed equally.
  • ,
  • Hiroshi Naito

      Affiliations

    • Institute for Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • These authors contributed equally.
  • ,
  • Malte Tiburcy

      Affiliations

    • Institute for Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • Department of Pharmacology, Heart Research Center Göttingen, Georg-August-University Göttingen, Germany
  • ,
  • Michael Didié

      Affiliations

    • Institute for Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • Department of Pharmacology, Heart Research Center Göttingen, Georg-August-University Göttingen, Germany
  • ,
  • Monika Nose

      Affiliations

    • Institute for Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • ,
  • Stephan Rosenkranz

      Affiliations

    • Klinik III für Innere Medizin, Universität zu Köln, Germany
    • Center for Molecular Medicine (CMMC), Universität zu Köln, Germany
    • Corresponding Author InformationCorrespondimg authors. Rosenkranz is to be contacted at Klinik III für Innere Medizin, Universität zu Köln, Germany. Zimmermann, University Medical Center Goettingen, Department of Pharmacology, Robert-Koch-Str. 40, 37075 Goettingen, Germany. Tel.: +49 551 39 5781; fax: +49 551 39 5699.
  • ,
  • Wolfram-H. Zimmermann

      Affiliations

    • Institute for Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • Department of Pharmacology, Heart Research Center Göttingen, Georg-August-University Göttingen, Germany
    • Corresponding Author InformationCorrespondimg authors. Rosenkranz is to be contacted at Klinik III für Innere Medizin, Universität zu Köln, Germany. Zimmermann, University Medical Center Goettingen, Department of Pharmacology, Robert-Koch-Str. 40, 37075 Goettingen, Germany. Tel.: +49 551 39 5781; fax: +49 551 39 5699.
    • Current address: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India - 721302.

Received 13 October 2009 ,Revised 26 February 2010 ,Accepted 15 March 2010.

References 

  1. Harrington EA, Bennett MR, Fanidi A, Evan GI. c-Myc-induced apoptosis in fibroblasts is inhibited by specific cytokines. EMBO J. 1994;13:3286–3295
  2. Romashkova JA, Makarov SS. NF-kappaB is a target of AKT in anti-apoptotic PDGF signalling. Nature. 1999;401:86–90
  3. Vantler M, Caglayan E, Zimmermann WH, Baumer AT, Rosenkranz S. Systematic evaluation of anti-apoptotic growth factor signaling in vascular smooth muscle cells. Only phosphatidylinositol 3′-kinase is important. J Biol Chem. 2005;280:14168–14176
  4. Vantler M, Huntgeburth M, Caglayan E, Ten Freyhaus H, Schnabel P, Rosenkranz S. PI3-kinase/Akt-dependent antiapoptotic signaling by the PDGF alpha receptor is negatively regulated by Src family kinases. FEBS Lett. 2006;580:6769–6776
  5. Rosenkranz S, Kazlauskas A. Evidence for distinct signaling properties and biological responses induced by the PDGF receptor alpha and beta subtypes. Growth Factors. 1999;16:201–216
  6. Sachinidis A, Gissel C, Nierhoff D, Hippler-Altenburg R, Sauer H, Wartenberg M, et al. Identification of plateled-derived growth factor-BB as cardiogenesis-inducing factor in mouse embryonic stem cells under serum-free conditions. Cell Physiol Biochem. 2003;13:423–429
  7. Hsieh PC, Davis ME, Gannon J, MacGillivray C, Lee RT. Controlled delivery of PDGF-BB for myocardial protection using injectable self-assembling peptide nanofibers. J Clin Invest. 2006;116:237–248
  8. Bishopric NH, Simpson PC, Ordahl CP. Induction of the skeletal alpha-actin gene in alpha 1-adrenoceptor-mediated hypertrophy of rat cardiac myocytes. J Clin Invest. 1987;80:1194–1199
  9. Naito H, Melnychenko I, Didie M, Schneiderbanger K, Schubert P, Rosenkranz S, et al. Optimizing engineered heart tissue for therapeutic applications as surrogate heart muscle. Circulation. 2006;114:I72–I78
  10. Zimmermann WH, Schneiderbanger K, Schubert P, Didie M, Munzel F, Heubach JF, et al. Tissue engineering of a differentiated cardiac muscle construct. Circ Res. 2002;90:223–230
  11. Communal C, Sumandea M, de Tombe P, Narula J, Solaro RJ, Hajjar RJ. Functional consequences of caspase activation in cardiac myocytes. Proc Natl Acad Sci U S A. 2002;99:6252–6256
  12. Zimmermann WH, Fink C, Kralisch D, Remmers U, Weil J, Eschenhagen T. Three-dimensional engineered heart tissue from neonatal rat cardiac myocytes. Biotechnol Bioeng. 2000;68:106–114
  13. Zimmermann WH, Didie M, Doker S, Melnychenko I, Naito H, Rogge C, et al. Heart muscle engineering: an update on cardiac muscle replacement therapy. Cardiovasc Res. 2006;71:419–429
  14. El-Armouche A, Singh J, Naito H, Wittkopper K, Didie M, Laatsch A, et al. Adenovirus-delivered short hairpin RNA targeting PKCalpha improves contractile function in reconstituted heart tissue. J Mol Cell Cardiol. 2007;43:371–376
  15. Eschenhagen T, Zimmermann WH, Kleber AG. Electrical coupling of cardiac myocyte cell sheets to the heart. Circ Res. 2006;98:573–575
  16. Radisic M, Park H, Chen F, Salazar-Lazzaro JE, Wang Y, Dennis R, et al. Biomimetic approach to cardiac tissue engineering: oxygen carriers and channeled scaffolds. Tissue Eng. 2006;12:2077–2091
  17. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001;25:402–408
  18. Kundra V, Escobedo JA, Kazlauskas A, Kim HK, Rhee SG, Williams LT, et al. Regulation of chemotaxis by the platelet-derived growth factor receptor-beta. Nature. 1994;367:474–476
  19. Valius M, Kazlauskas A. Phospholipase C-gamma 1 and phosphatidylinositol 3 kinase are the downstream mediators of the PDGF receptor's mitogenic signal. Cell. 1993;73:321–334
  20. Armstrong MT, Lee DY, Armstrong PB. Regulation of proliferation of the fetal myocardium. Dev Dyn. 2000;219:226–236
  21. Ponten A, Folestad EB, Pietras K, Eriksson U. Platelet-derived growth factor D induces cardiac fibrosis and proliferation of vascular smooth muscle cells in heart-specific transgenic mice. Circ Res. 2005;97:1036–1045
  22. Ponten A, Li X, Thoren P, Aase K, Sjoblom T, Ostman A, et al. Transgenic overexpression of platelet-derived growth factor-C in the mouse heart induces cardiac fibrosis, hypertrophy, and dilated cardiomyopathy. Am J Pathol. 2003;163:673–682

PII: S0022-2828(10)00093-3

doi: 10.1016/j.yjmcc.2010.03.008

Journal of Molecular and Cellular Cardiology
Volume 48, Issue 6 , Pages 1316-1323 , June 2010