Journal of Molecular and Cellular Cardiology
Volume 47, Issue 6 , Pages 781-788 , December 2009

Carbamylated darbepoetin derivative prevents endothelial progenitor cell damage with no effect on angiogenesis

  • Rafael Ramirez

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • Corresponding Author InformationCorresponding author. Tel.: +34 957 010452; fax: +34 957 010452.
    • ,
  • Julia Carracedo

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Sonia Nogueras

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Paula Buendia

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Ana Merino

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Sagrario Cañadillas

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Mariano Rodríguez

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Ciro Tetta

      Affiliations

    • Department of Medicine, University of Cordoba, Cordoba, Spain
    • ,
  • Alejandro Martin-Malo

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain
    • ,
  • Pedro Aljama

      Affiliations

    • Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Avda. Menéndez Pidal S/N, Cordoba-14004, Spain

Received 27 April 2009 ,Revised 3 September 2009 ,Accepted 9 September 2009.

References 

  1. Winearls CG, Oliver DO, Pippard MJ, Reid C, Downing MR, Cotes PM. Effect of human erythropoietin derived from recombinant DNA on the anaemia of patients maintained by chronic haemodialysis. Lancet. 1986;22:1175–1178
  2. Ghezzi P, Brines M. Erythropoietin as an antiapoptotic, tissue-protective cytokine. Cell Death Differ. 2004;11:S37–S44
  3. Brines M, Cerami A. Erythropoietin-mediated tissue protection: reducing collateral damage from the primary injury response. J. Intern. Med. 2008;264:405–432
  4. Bao H, Jacobs-Helber SM, Lawson AE, Penta K, Wickrema A, Sawyer ST. Protein kinase B (c-Akt), phosphatidylinositol 3-kinase, and STAT5 are activated by erythropoietin (EPO) in HCD57 erythroid cells but are constitutively active in an EPO-independent, apoptosis-resistant subclone (HCD57-SREI cells). Blood. 1999;93:3757–3773
  5. Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, et al. Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat. Med. 1999;4:434–438
  6. Geft D, Schwartzenberg S, George J. Circulating endothelial progenitor cells in cardiovascular disorders. Expert Rev. Cardiovasc. Ther. 2008;8:1115–1121
  7. Verma S, Kuliszewski MA, Li SH, Szmitko PE, Zucco L, Wang CH, et al. C-reactive protein attenuates endothelial progenitor cell survival, differentiation, and function: further evidence of a mechanistic link between C-reactive protein and cardiovascular disease. Circulation. 2004;109:2058–2067
  8. Ramirez R, Carracedo J, Merino A, Nogueras S, Alvarez-Lara MA, Rodríguez M, et al. Microinflammation induces endothelial damage in hemodialysis patients: the role of convective transport. Kidney Int. 2007;72:108–113
  9. Tousoulis D, Andreou I, Antoniades C, Tentolouris C, Stefanadis C. Role of inflammation and oxidative stress in endothelial progenitor cell function and mobilization: therapeutic implications for cardiovascular diseases. Atherosclerosis. 2008;201:236–247
  10. Heeschen C, Aicher A, Lehmann R, Fichtlscherer S, Vasa M, Urbich C, et al. Erythropoietin is a potent physiologic stimulus for endothelial progenitor cell mobilization. Blood. 2003;102:1340–1346
  11. Bahlmann FH, DeGroot K, Duckert T, Niemczyk E, Bahlmann E, Boehm SM, et al. Endothelial progenitor cell proliferation and differentiation is regulated by erythropoietin. Kidney Int. 2003;64:1648–1652
  12. de Groot K, Bahlmann FH, Sowa J, Koenig J, Menne J, Haller H, et al. Uremia causes endothelial progenitor cell deficiency. Kidney Int. 2004;66:641–646
  13. Ramirez R, Martin-Malo A, Aljama P. Inflammation and hemodiafiltration. Contrib. Nephrol. 2007;158:210–215
  14. Drüeke TB, Locatelli F, Clyne N, Eckardt KU, Macdougall IC, Tsakiris D, et al. Normalization of hemoglobin level in patients with chronic kidney disease and anemia. N. Engl. J. Med. 2006;355:2071–2084
  15. Leist M, Ghezzi P, Grasso G, Bianchi R, Villa P, Fratelli M, et al. Derivatives of erythropoietin that are tissue protective but not erythropoietic. Science. 2004;305:239–242
  16. Brines M, Grasso G, Fiordaliso F, Sfacteria A, Ghezzi P, Fratelli M, et al. Erythropoietin mediates tissue protection through an erythropoietin and common beta-subunit heteroreceptor. Proc. Natl. Acad. Sci. U. S. A. 2004;101:14907–14912
  17. Komatsu N, Yamamoto M, Fujita H, Miwa A, Hatake K, Endo T, et al. Establishment and characterization of an erythropoietin-dependent subline, UT-7/Epo, derived from human leukemia cell line, UT-7. Blood. 1993;82:456–464
  18. Ratajczak J, Majka M, Kijowski J, Baj M, Pan ZK, Marquez LA, et al. Biological significance of MAPK, AKT and JAK-STAT protein activation by various erythropoietic factors in normal human early erythroid cells. Br. J. Haematol. 2001;115:195–204
  19. Andrews NC, Faller DV. A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res. 1991;19:2499–2507
  20. Hill HM, Zalos G, Halcox JPJ, Schenke WH, Wacliwiw MA, Quyyumi AA, et al. Circulating endothelial progenitor cells, vascular function and cardiovascular risk. N. Engl. J. Med. 2003;348:593–600
  21. Teoh ML, Turner PV, Evans DH. Tumorigenic poxviruses up-regulate intracellular superoxide to inhibit apoptosis and promote cell proliferation. J. Virol. 2005;79:799–811
  22. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. U. S. A. 1995;92:9363–9367
  23. Ramirez R, Carracedo J, Jimenez R, Canela A, Herrera E, Aljama P. Massive telomere loss is an early event of DNA damage-induced apoptosis. J. Biol. Chem. 2003;278:836–842
  24. Farrell F, Lee A. The erythropoietin receptor and its expression in tumor cells and other tissues. Oncologist. 2004;9(Suppl 5):18–30
  25. Asahara T. Cell therapy and gene therapy using endothelial progenitor cells for vascular regeneration. Handb. Exp. Pharmacol. 2007;180:181–194
  26. Ceradini DJ, Kulkarni AR, Callaghan MJ, Tepper OM, Bastidas N, Kleinman ME, et al. Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1. Nat. Med. 2004;10:858–864
  27. Asahara T, Takahashi T, Masuda H, Kalka C, Chen D, Iwaguro H, et al. VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells. EMBO J. 1999;18:3964–3972
  28. Jujo K, Ii M, Losordo DW. Endothelial progenitor cells in neovascularization of infarcted myocardium. J. Mol. Cell. Cardiol. 2008;45:530–544
  29. López-Gómez JM, Portolés JM, Aljama P. Factors that condition the response to erythropoietin in patients on hemodialysis. and their relation to mortality. Kidney Int. 2008;111 Suppl:75–81

PII: S0022-2828(09)00381-2

doi: 10.1016/j.yjmcc.2009.09.005

Journal of Molecular and Cellular Cardiology
Volume 47, Issue 6 , Pages 781-788 , December 2009

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